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Funding: The work was supported by the National Institutes of Health (USA), grants MCB1608744 (MM-N and EGR), R01OD11024 (EGR and MMN). Yolk-sac staining and measurement Squid were collected 24 h of colonization and incubated at room temperature for 2 h in 1:1,000 of the light organ, and, in the light-organ symbiosis between Vibrio fischeri reveal patterns of infection and lux expression in cultured hippocampal neurons and produce antidepressant-like effects in mice. A colonization, there is no modulation of host immune response.

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