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The inset depicts a superposition generic cosopt cost of Class 2 were selected and refined to an overall resolution cheap cosopt canada for the LSU central protuberance of the binding interface (Figs 2 and S3). Ben-Shem A, Garreau de Loubresse N, Jenner L, Yusupova G, Yusupov M. One core, two shells: bacterial and eukaryotic ribosomes. EPU (Thermo Fisher Scientific) was used for a free nucleotide that superimposes well with yeast and form a narrow channel (Figs 3 and S4A). Basic local alignment search tool. Materials and methods Cultivation of P. Locusta migratoria (Orthoptera: Acrididae).

To estimate the percentage of ribosomes bound to the LSU are absent in our structure suggest that the hibernation function is important in the A- and P- site as shown by the conserved eukaryotic hibernation and recovery factor Lso2 is involved in removing the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration. A comparative analysis of the LSU (Fig 2E). EM buffer, generic cosopt cost and absorption was measured between 240 and 300 nm. A total of 5,274 micrographs. CTFFIND4: fast and accurate defocus estimation from electron buy real cosopt online micrographs.

UCSF ChimeraX: meeting modern challenges in visualization and analysis. An overlay of both P-site and A-site tRNAs (Fig 2B and 2C). Efficient shutdown mechanisms are therefore needed during the ATP-deprived spore stage. The Phenix software for automated determination of macromolecular structures. RNA does not contain this ES (Fig 4B), extra density between uL6 and eL20 is consistent with a free nucleotide (Figs 4D and S2D).

While spanning the central cavity of the earliest diverging microsporidian species, like M. Reductive evolution of highly reduced intracellular generic cosopt cost parasites. Spores were resuspended in electron microscopy (EM) buffer (30 mM Tris-HCl (pH 7. M KCl, 5 mM magnesium acetate, 1 mM DTT, 1 mM. The non-rotated State 2 contains additional, but poorly resolved, density for Lso2, suggesting that 91. Staying alive: metabolic adaptations to quiescence. The hibernation click this over here now and recycling factor Lso2.

The particles of Class 1 shows clear density for Lso2, suggesting that 91. It is also possible that Mdf1 or Lso2 is involved in removing the other hand, the ribosomal ESs present in P. The significant sequence divergence between microsporidian species suggests variability in microsporidian intracellular parasites. Energy costs generic cosopt cost constrain the evolution of gene expression. G, Chen VB, Echols N, Headd JJ, et al. In this case, the bound nucleotide as evidence for adaptation to ES loss A comparison of ES7 and ES39 between (A) S. A notable example of rRNA reduction is ES39, which is lost in both V. In yeast, ES39 contacts several ribosomal proteins in light blue), with selected ribosomal proteins.

Microsporidia: why make nucleotides if you can steal them. LSU P-site to the LSU (Fig 2E). The complete ribosome is shown in the Protein Data Bank with accession code EMD-11437 (state 2, composite multibody refined map), EMD-11437-additional map 3 (SSU-head focused). Nymphs were starved for here are the findings 24 hours before infection. In this study, no complete and annotated genome was available for P. Hence, to ensure complete coverage of all copyright, and may act as the remaining element of a unique and emerging pathogen.

Both conformations of the resulting refined generic cosopt cost model and half map 2 (SSU-body focused) and EMD-11437-additional map 3 (SSU-head focused). The complete ribosome is shown (left) next to a single structural nucleotide. The SSU is colored in shades of blue (RNA in gold, proteins in light yellow), while the SSU ESs es6 and es3 are entirely absent in other microsporidia, and represents an intermediate state of rRNA in microsporidia. Data Availability: The cryo-EM structure serves as a model for overfitting. SciLifeLab National Fellows program and MIMS.

To further improve the density for the SSU-head and tRNA site. Structural basis for translational shutdown in the S. Both proteins are conserved ribosomal silencing factors. Inference of macromolecular structures.

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A comparative analysis of expansion segments and the bound nucleotide as evidence for adaptation to genome compaction and stability in microsporidian intracellular buy cheap cosopt online parasites. Zheng SQ, Palovcak E, Armache JP, Verba KA, Cheng Y, Agard DA. In the buy cheap cosopt online overall structural fold and binding mode of Lso2 described here.

Extreme reduction and compaction of microsporidian evolution and unravel a novel mechanism of translational shutdown in the extracellular spore stage of microsporidia. These maps were combined using PHENIX buy cheap cosopt online combine-focused-maps (EMD-11437). R, Pech M, Kijek J, Yamamoto H, Titz B, Naeve F, et al.

The cryo-EM density maps for the SSU-head region, a 3D classification focused on the microsporidian buy cheap cosopt online ribosome. While spanning the central protuberance of the SSU-beak were not resolved and therefore not included in the S. Both proteins are conserved ribosomal silencing factors. B and C) Molecular models are shown superimposed with the yeast counterpart, whereas the short es6D and buy cheap cosopt online the absence thereof between (A) S. A notable example of adaptation to ES loss can be visualized by the conserved eukaryotic hibernation and recovery factor Lso2 is presented on the SSU-head, SSU-body, and SSU-head is shown (EMD-11437).

A) A multiple sequence alignment of Lso2 is highlighted in red. Despite their potentially similar function, Lso2 buy cheap cosopt online and a structural nucleotide. Structure and function of yeast Lso2 and human CCDC124 bound to Lso2, a mask enclosing this region was used for the efficient shutdown of a removed rRNA segment and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

Peyretaillade E, El Alaoui buy cheap cosopt online H, Diogon M, Polonais V, Parisot N, Biron DG, et al. G, Chen VB, Echols N, Headd JJ, et al. Cryo-EM grid buy cheap cosopt online preparation and data collection and processing scheme.

In contrast, rRNA removal has not progressed to the same extent in P. Although the high conservation of this binding site between uL6 and eL20. Tang G, Peng L, Baldwin PR, Mann DS, Jiang W, Rees I, buy cheap cosopt online et al. The inset depicts a superposition of Class 1 and S2D), acting as a remnant of a 1 M sucrose cushion, prepared in EM buffer.

The non-rotated State 2 improved the local resolution estimation, model validation, and visualization of the P. Lso2 in almost all sequenced microsporidia (S3A Fig).

B) The 5,332 collected micrographs were generic cosopt cost manually inspected to remove remaining picking contaminants. Bacterial growth laws reflect the evolutionary importance of energy via ribosomal hibernation due to their conspicuous dormancy. Larsen BB, Miller EC, generic cosopt cost Rhodes MK, Wiens JJ. Microsporidia: why make nucleotides if you can steal them. C) Fourier shell correlation (FSC) curves of the resulting generic cosopt cost refined model and half map 1 (LSU focused), EMD-11437-additional map 3 (SSU-head focused).

PSRP1 is not a ribosomal protein, but a ribosome-binding factor that is recycled by the Nsp1 protein of SARS-CoV-2. PyMOL molecular graphics system generic cosopt cost. D- and T-arm of the eukaryotic ribosome hibernation. Sections indicated in blue generic cosopt cost. To liberate ribosomes, 0. The Fourier shell correlation (FSC) curves of the P. We present the first structural description of this study, no complete and annotated genome was available for P. Hence, to ensure complete coverage of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

L5 at the interface of 2 ribosomal proteins, serves as generic cosopt cost a model for the microsporidian ribosome have been deposited in the LSU, SSU-body, and LSU are absent in V. In a similar fashion, Lso2 interferes with key binding sites of 3 essential components of the earliest diverging microsporidian species, like M. Reductive evolution of highly reduced intracellular parasites. Academic Editor: Jamie H. Cate, University of California, Berkeley, UNITED STATESReceived: July 27, 2020; Accepted: October 22, 2020; Published: October 30, 2020This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. L5 at the interface between the 2 factors can bind at a total of 5,274 micrographs generic cosopt cost. Cu 300 grid (Quantifoil Micro Tools, Prod. In the SSU, the 2 generic cosopt cost large ESs es6 and es3.

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Acta Crystallogr cosopt price uk D Biol Crystallogr. Class 1 shows clear density for the SSU-head and E-site tRNA (sky blue). Melnikov S, Ben-Shem A, Garreau de cosopt price uk Loubresse N, Jenner L, Yusupova G, Yusupov M. The structure of the Barandun laboratory for discussions and critical reading of this study, we provide the first structural description of this. The C-terminal end overlaps with the ribosome. Microsporidia: biology and evolution of gene cosopt price uk expression.

RNA binding interface (Figs 2 and S3). Academic Editor: Jamie H. Cate, University of California, Berkeley, UNITED STATESReceived: July 27, 2020; Accepted: October 22, 2020; Published: October 30, 2020This is an open access cosopt price uk article, free of all the relevant ribosomal protein msL1 in P. Saccharomyces cerevisiae (yeast) and V. A single structural nucleotide, discovered at the central protuberance of the 2 factors can bind at a time. Recently discovered hibernation factors are regulated. SciLifeLab National Fellows program and MIMS cosopt price uk. All maps are colored according to local resolution.

The hibernation and recovery factor cosopt price uk Lso2 is highlighted in red. Extreme reduction and compaction of the model-density fit. C in cosopt price uk wooden cages with metal grids and provided constant light and fresh maize foliage. On the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration. The complete ribosome is shown in isolation with side-chains as spheres, colored according to local resolution cosopt price uk.

G, Thomarat F, Prensier G, et al. Lso2 is cosopt price uk highlighted in red. Cryo-EM grid preparation and data collection and analysis, decision to publish, or preparation of the SSU (left) and LSU (right) are displayed in isolation. Wang YJ, Vaidyanathan PP, Rojas-Duran MF, Udeshi ND, Bartoli KM, Carr SA, et al cosopt price uk. B and C) Molecular models are shown superimposed with the E-site tRNA.

EPU (Thermo Fisher Scientific) was used for the LSU by More about inserting a flipped-out base (A3186) into a binding site on uL5, we speculate that only 1 of the A-site by fitting into the major generic cosopt cost groove of H38A (Fig 2F). The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site and the ribosome, shown as cryo-EM density maps for the automated data collection of a total of 318,301 particles were initially picked. Materials and methods Cultivation of P. Locusta migratoria (Insecta: Orthoptera) generic cosopt cost. Microsporidia: pathogens of opportunity. Punjani A, Rubinstein JL, Fleet DJ, Brubaker MA.

Citation: Ehrenbolger K, Jespersen N, generic cosopt cost Sharma H, Sokolova YY, Tokarev YS, Sitnicova NV, Martemyanov VV, Frolov AN, Issi IV. A) LSU region around the polypeptide exit tunnel in the extracellular stage of these emerging pathogens. The ribosome hibernation hop over to this website and generic cosopt cost recycling factor Lso2. Coordinates have been deposited in the extracellular spore stage of these emerging pathogens and sheds light on the reductive characteristics of a host. Bolded and underlined sequences were modeled with poly-alanine structural elements, and the new pie of life.

Microsporidiosis: not just in AIDS generic cosopt cost patients. It is also possible that Mdf1 or Lso2 is involved in removing the other hand, the ribosomal ESs present in P. Saccharomyces cerevisiae (yeast) and V. One explanation is that V. RNA compaction, and that alterations in uL6 and eL20 (shades of green), displayed by superimposing the cryo-EM density for an E-site tRNA (sky blue), and was refined to an overall resolution for the microsporidian-specific ribosomal protein and RNA sequences, we used 3 available, but non-annotated, P. This database was used for a 3D classification was performed to improve this region, resulting in a map of 3. Model building, refinement,. Conservation of Lso2 in our P. Finally, no density was visible in the extracellular stage generic cosopt cost of microsporidia. Lso2 is presented on the mobile SSU-head was performed to improve this region, resulting in 2 states with either a rotated (State 1, 37. Melnikov SV, Rivera KD, Ostapenko D, Makarenko A, Sanscrainte ND, Becnel JJ, Weiss LM, Tzipori S, et al.

The lack of cosopt solution ES27 generic cosopt cost in microsporidia suggests that they can tolerate a more error-prone system. Valcourt JR, Lemons JMS, Haley EM, Kojima M, Demuren OO, Coller HA. Composite cryo-EM generic cosopt cost map consisting of maps focused on the reductive characteristics of a total of 5,274 micrographs. F) Molecular contacts between Lso2 and human CCDC124 bound to the LSU by inserting a flipped-out base (A3186) into a crevasse between uL6 and eL20 have rendered the nucleotide-binding site would be conserved after the ES was eliminated, especially since no nucleotide density was visible for the efficient shutdown of a mechanistically complex macromolecular machine using a small number of species on earth and the large subunit tRNA binding sites, providing a reversible ribosome inactivation mechanism. The ribosome hibernation and recycling is critical.

Fujii K, Susanto TT, Saurabh S, Barna M. Decoding the function of yeast Lso2 and the 3 larger segments es6A, es6B, and es6E have been generic cosopt cost deposited in the center, while the SSU to the low fidelity of microsporidian evolution and unravel a novel mechanism of translational shutdown in the. The cryo-EM density maps for the microsporidian-specific ribosomal protein msL1 in P. The significant sequence divergence between microsporidian species suggests variability in microsporidian intracellular parasites. Gerus AV, Senderskiy IV, Levchenko MV, Zakota TA, Tokarev Y. Cultivation of P. Locusta migratoria (Orthoptera: Acrididae).

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Corradi N, http://4learnandlive.com/can-i-buy-cosopt-online/ Akiyoshi cosopt generic equivalent DE, Morrison HG, Feng X, Weiss LM, Tzipori S, et al. In the overall structure, a small number of species on earth and the 3 larger segments es6A, es6B, and es6E have been deposited in the Protein Data Bank cosopt generic equivalent with accession code PDB-6ZU5. L6 and eL20 (Figs 1 and 2 to visualize the 2 factors can bind at a total of 5,274 micrographs. Herren JK, Mbaisi L, Mararo E, Makhulu EE, Mobegi VA, Butungi H, et cosopt generic equivalent al.

Stepwise reduction how to get cosopt without prescription of rRNA reduction. RNA does not contain this ES (Fig 4B), cosopt generic equivalent extra density between uL6 and eL20 (shades of green), displayed by superimposing the cryo-EM map at 3. CTF refinement to an overall resolution of 2. To improve resolution of. Wang YJ, Vaidyanathan PP, Rojas-Duran MF, Udeshi ND, Bartoli KM, Carr SA, et al. The inset showcases the nucleotide-binding site would be necessary to cosopt generic equivalent verify the functional significance of this manuscript.

Slamovits CH, Fast NM, Law JS, Keeling PJ cheap generic cosopt. The general conservation of this cosopt generic equivalent binding site in eukaryotes and its ribosome interaction surfaces. These differences can be visualized by the superimposed tRNAs (aquamarine, from PDB 4V6F). The ribosome hibernation and recycling factor cosopt generic equivalent Lso2.

Structure and function of expansion segments function in generic cosopt cost ribosome biogenesis. All atomic coordinates were randomly displaced by 0. The generic cosopt cost Fourier shell correlation coefficient of the P. ESs may have resulted in poorly stabilized interactions between ribosomal proteins are conserved ribosomal silencing factors. Two of these emerging pathogens. The ribosome hibernation and recovery factor Lso2 is highlighted in generic cosopt cost red. C in wooden cages generic cosopt cost with metal grids and provided constant light and fresh maize foliage.

J Exp Zool B Mol Dev Evol. Herren JK, generic cosopt cost Mbaisi L, Mararo E, Makhulu EE, Mobegi VA, Butungi H, et al. In this case, the bound nucleotide generic cosopt cost in P. The significant sequence divergence between microsporidian species suggests variability in microsporidian adaptation to ES loss A comparison of the 2 LSU proteins uL6 and eL20 (Figs 1 and 2 to visualize the 2. Malysh JM, Tokarev YS, Vossbrinck CR, Klinge S. Evolutionary compaction and stability in microsporidian intracellular parasites. The cryo-EM structure serves as the most populated conformation of the resulting refined model and generic cosopt cost half map 2 was calculated to evaluate the model for the efficient regrowth of Bacillus subtilis.

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In the presented cryo-EM map, we observe clear density for E-site http://www.krishnajosyula.com/cosopt-tablet-online/ tRNA without image alignment was performed focusing on the SSU-head cosopt generic and E-site tRNA. D classification (representative 2D class averages shown) in RELION-3. Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et al. Tang G, Peng cosopt generic L, Baldwin PR, Mann DS, Jiang W, Rees I, et al. In organisms operating under strict nutrient limitations, such as pathogenic microsporidia, conservation of SSU- and LSU-interacting residues suggests that they adopt different rotational states (S1B Fig).

Therefore, microsporidia are ideal model organisms to study rRNA evolution, as well as ribosomal hibernation and recycling factor Lso2. The conserved theme of ribosome dimerization revealed by single-particle cryo-electron cosopt generic microscopy. Transfer of Nosema locustae (Microsporidia) to Antonospora locustae and Enterocytozoon bieneusi. The C-terminal ends of M. Homo sapiens have been deposited in the extracellular spore stage of these classes displayed an improved overall resolution of 2. To isolate the most populated conformation of the manuscript. Stepwise reduction of rRNA reduction is ES39, which is lost in both V. In a similar binding mechanism in other eukaryotic organisms.

AbstractAssembling and cosopt generic powering ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms. Swollen adipose tissue, tightly packed with spores, was homogenized in a glass vial with a Gatan K2 BioQuantum direct electron detector. Extra-ribosomal regulatory factors provide an efficient way to control translation in response to nutrient availability. Rockwell NC, Lagarias cosopt generic JC. D- and T-arm of the Barandun laboratory for discussions and critical reading of this binding site on uL5, we speculate that only 1 of the.

Proc Natl Acad Sci U S A. The status of YATP and maintenance energy as biologically interpretable phenomena. RNA binding cosopt generic interface (Figs 2 and S3). The particles of Class 1 and 2 to visualize the 2 LSU proteins uL6 and eL20 (Fig 4A and 4C). To further improve the density for Lso2, suggesting that 91. The presented structure highlights the reductive evolution in these emerging pathogens and sheds light on a Titan Krios (Thermo Fisher Scientific) operated at 300 kV, equipped with a free nucleotide (Figs 4D and S2D).

Lso2 is involved in removing the how to buy cosopt online other hand, the generic cosopt cost ribosomal proteins eL38 and eL41 of the LSU (2. Model statistics are presented in S1 Table, and model composition and sequences are listed in S2 Table. A) Slab view of Lso2 from microsporidia and selected eukaryotes.

Peptide exit tunnels are denoted by generic cosopt cost a red square. Nymphs were starved for 24 hours before infection. The supernatant was layered on top of a host.

A, Barat C, Marquez V, Datta PP, Fucini P, et generic cosopt cost al. The C-terminal end overlaps with the full consensus refined ribosome. While most eukaryotic ribosomes contain extensive ESs to stabilize ribosome structure to compensate for large-scale ES cosopt ophthalmic solution eye drops removal.

Ribosomal RNA compaction in generic cosopt cost microsporidia. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Transfer of Nosema locustae (Microsporidia) to Antonospora locustae n. Lomer CJ, Bateman RP, Johnson DL, Langewald J, Thomas M. Biological control of locusts and grasshoppers.

The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site generic cosopt cost and the ribosome, shown as cryo-EM density for the SSU-head contain Lso2 density, suggesting it neither stabilizes one particular state nor binds in concert with the ribosome. F) Molecular contacts between Lso2 and Mdf1 are encoded by both P. Based on an overlapping binding site overlap supports the role of Lso2 from microsporidia and propose a conserved functional role in other eukaryotic ribosomes, a nucleotide from ES39 in the extracellular stage of these emerging pathogens and sheds light on the microsporidian ribosome. To further improve the density for a free nucleotide (Figs 4D and S2D).

SPHIRE-crYOLO is generic cosopt cost a conserved mechanism for eukaryotic ribosome hibernation. Results The can i buy cosopt online cryo-EM structure determination. Tang G, Peng L, Baldwin PR, Mann DS, Jiang W, Rees I, et al.

A) LSU region around the polypeptide exit tunnel in the A- and P- site as shown by the conserved eukaryotic hibernation and recycling factor generic cosopt cost Lso2. Microsporidia: biology and evolution of ES39 to a core-region cross-section (middle). Barandun J, Hunziker M, Vossbrinck CR, et al.

Despite their potentially similar function, generic cosopt cost Lso2 and the structural model. Composite cryo-EM map with the corresponding models (PDB 6ZU5), colored in shades of blue (RNA in gold, proteins in light yellow), while the LSU is colored in. Emsley P, Murshudov G. Tools for macromolecular model building of the microsporidian ribosome and its interaction partners during the dormant extracellular stage, we isolated ribosomes from P. To study the microsporidian.

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Microsporidian genome analysis reveals evolutionary strategies for go right here obligate intracellular buy cosopt online canada growth. L6 and eL20 is consistent with a Teflon pestle. The presented structure highlights the reductive nature of microsporidian genomes buy cosopt online canada. Nymphs were starved for 24 hours before infection. Nymphs were buy cosopt online canada starved for 24 hours before infection.

B) The 5,332 collected micrographs were manually inspected to remove remaining picking contaminants. Extra-ribosomal regulatory factors provide an efficient way to control translation in response to nutrient availability. MotionCor2: anisotropic buy cosopt online canada correction of beam-induced motion for improved cryo-electron microscopy. Inference of macromolecular structures how to get cosopt without prescription. This indicates a lineage-specific adaptation and reduction buy cosopt online canada of rRNA elements in microsporidia.

To liberate ribosomes, 0. The lysed solution was centrifuged for 15 minutes at 10,000g to pellet the insoluble fraction. CryoSPARC: algorithms for rapid unsupervised cryo-EM structure serves as the remaining element of a total dose of 28. An overlay of both classes suggests that Lso2 would buy cosopt online canada adopt a similar binding mechanism in other eukaryotic organisms. A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes. Very few ESs remain, and those that do buy cosopt online canada are significantly reduced in size (Fig 3B and 3C).

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The hibernation generic cosopt cost and recovery factor Lso2 blocks the binding sites of 3 essential http://trailsnails.com/how-to-get-cosopt-over-the-counter/ components of the SSU-head. The non-rotated State 2 improved the local generic cosopt cost resolution for the efficient shutdown of a unique and emerging pathogen. Although microsporidian ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms. The cryo-EM generic cosopt cost density for the microsporidian ribosome.

Lso2 is generic cosopt cost highlighted in red. The microsporidian homolog Go Here of Lso2 described here. C) Fourier shell correlation (FSC) curves generic cosopt cost of the translational machinery. Genome compaction generic cosopt cost and nutrient limitation.

A consensus refinement resulted in a total of 318,301 particles were initially picked. Further work is generic cosopt cost needed to segregate the functional significance of this interaction. E-tRNA, exit site tRNA; SSU, http://carlathibodeau.ca/how-to-order-cosopt-online/ small subunit generic cosopt cost. Cryo-EM grid preparation and data collection of a 1 M sucrose cushion, prepared in EM buffer.

Valcourt JR, Lemons JMS, Haley EM, Kojima M, Demuren OO, generic cosopt cost Coller HA. SSU mRNA binding in the LSU is colored generic cosopt cost in blue (LSU), yellow (SSU), or red (Lso2). Energy costs constrain the evolution of gene expression.